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The route from Prague to Rome is served daily by 8 bus departing from the stations of Prague Florenc Central Bus Station, ÚAN Florenc, Prague ÚAN Florenc, Prague Anděl and arriving to the stations of Rome Tiburtina, Via Teodorico, Rome Tiburtina Bus Station.
The average duration of the journey is 37 h 45 min. The first bus leaves at 16:30. The last is at 20:00.

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