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The route from Rome to Prague is served daily by 16 bus departing from the stations of Rome Tiburtina Bus Station, Rome Tiburtina, Via Teodorico, Rome Anagnina and arriving to the stations of Prague Main Railway Station, Prague ÚAN Florenc, Prague Anděl, Prague Florenc Central Bus Station, ÚAN Florenc, Prague Wilsonova Bus Station.
The average duration of the journey is 21 h 27 min. The first bus leaves at 09:45. The last is at 23:45.

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