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The route from Prague to Berlin is served daily by 181 bus departing from the stations of Prague Main Railway Station, Prague Florenc Central Bus Station, ÚAN Florenc, Prague ÚAN Florenc, Airport PRG Letiště Václava Havla, Prague Airport Terminal 1/2 and arriving to the stations of Berlin Central Bus Station, Berlin TXL, E, Berlin Südkreuz, Berlin SXF, Südkreuz, Berlin Schönefeld Airport, Schönefeld SXF Airport, ZOB, Berlin Alexanderplatz, Berlin S-Treptower Park, Berlin Alt-Tegel, Berlin Zoo.
The average duration of the journey is 4 h 40 min. The first bus leaves at 00:55. The last is at 23:40.

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