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The route from Berlin to Prague is served daily by 101 bus departing from the stations of Berlin Central Bus Station, Berlin Südkreuz, Berlin Alexanderplatz, Berlin S-Treptower Park, Berlin SXF, Berlin TXL, E, ZOB, Südkreuz, Schönefeld SXF Airport and arriving to the stations of Prague Florenc Central Bus Station, Prague Main Railway Station, Prague ÚAN Florenc, Airport PRG Letiště Václava Havla, ÚAN Florenc.
The average duration of the journey is 4 h 42 min. The first bus leaves at 07:30. The last is at 23:40.

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