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The route from Prague to Hamburg is served daily by 21 bus departing from the stations of Prague ÚAN Florenc, Prague Main Railway Station, ÚAN Florenc, Prague Florenc Central Bus Station, Prague Anděl and arriving to the stations of Hamburg Airport, Hamburg Harburg, Hamburg, Zentral Omnibus Bahnhof, Hamburg Central Bus Station.
The average duration of the journey is 10 h 47 min. The first bus leaves at 10:10. The last is at 23:40.

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